I have to treat mitochondria with rotenone in aiming to induce Reactive oxygen species (ROS) and by antioxidant prevents it's ROS generation.

Therefore,by In-vitro model how can I take the volume needed for mitochondrial treatment. If we know their molar concentration only?

For example

1)Control- (mitochondria)

2) Mitochondria+ rotenone+ antioxidant.

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