I have to treat mitochondria with rotenone in aiming to induce Reactive oxygen species (ROS) and by antioxidant prevents it's ROS generation.
Therefore,by In-vitro model how can I take the volume needed for mitochondrial treatment. If we know their molar concentration only?
For example
1)Control- (mitochondria)
2) Mitochondria+ rotenone+ antioxidant.