Snehal Kadam

5 Questions 8 Answers 0 Followers

Questions related from Snehal Kadam

How to decide the concentration need to induce oxidative stress in isolated mitochondria of rat brain by using either rotenone & sodium nitroprusside?

I'm trying to induce oxidative stress by using rotenone and SNP sodium nitroprusside in isolated mitochondria of rat brain. This is to study GSH assay from control (Mitochondria) group in...

12 June 2023 6,234 2 View

How to calculate the GSH present in mitochondrial protein in umol/gm?

While doing GSH on rat mitochondria, I have calculated protein concentration by Bradford's assay (1.35mg in 2ml buffer). In case of GSH assay I got concentration of 0.23mM (GSH) in mitochondria....

04 May 2023 2,797 6 View

How to treat mitochondria in-vitro by antioxidant and ROS inducer (rotenone) to study lipid peroxidation and GSH Assay?

I have to treat mitochondria with rotenone in aiming to induce Reactive oxygen species (ROS) and by antioxidant prevents it's ROS generation. Therefore,by In-vitro model how can I take the volume...

08 February 2023 6,351 4 View

What is the Methodology for calibration curve of rotenone?

Rotenone is the insecticide used as ROS Inducer in mitochondria. In aiming that what will be the method for the preparation of calibration curve for rotenone?

08 February 2023 5,462 0 View

How to treat mitochondria in-vitro by antioxidant and ROS inducer (rotenone) to study lipid peroxidation and GSH Assay?

I have to treat mitochondria with rotenone in aiming to induce Reactive oxygen species (ROS) and by antioxidant prevents it's ROS generation. Therefore,by In-vitro model how can I take the volume...

08 February 2023 2,850 0 View