I am currently doing invasion assay. I have fixed the matrigel coated inserts after 20 h of incubation with H1299 cells in 2% PFA and stained with 0.5% crystal violet. I have air dried them overnight. For how long I can store them like this at room temperature? What is the best method to store them?
The inserts can be stored for a long time after provided it was properly fixed and air-dried. Just store them in room-temperature in a culture plate. I stored my inserts in 24 well plates for a long time without any problems.
The inserts can be stored in 24-wells plates for extended time (years) at room temperature without loss of color if you keep them in the dark. The crystal violet tend to fade with light exposure.
Dear Saba,
I totally agree with above comments, we use to keep the inserts for weeks in 24-wells plates at room temp before we carry out the experiments, without any problem.
Dear Saba,
Make sure that you use filter sterilized fixative, and stains to minimize the growth of fungi in the inserts.
Best
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