Saba Tabasum

4 Questions 10 Answers 0 Followers

Questions related from Saba Tabasum

How to induce chemical hypoxia with CoCl2 in A549 cells?

I would like to know the exact protocol to induce chemical hypoxia in A549 cells. There are some variations that I have noticed in the method in various papers which are confusing. Do we starve...

19 June 2016 9,005 3 View

How to starve HaCat cells for wound healing assay?

I normally starve cells for 24 hours in serum free media and then add my drug in serum media, scratch and add this treatment media and follow the healing. In case of HaCat I starved the cell in 1%...

23 April 2016 1,010 3 View

How to store matrigel coated culture inserts after processing? Can they be stored at room temperature?

I am currently doing invasion assay. I have fixed the matrigel coated inserts after 20 h of incubation with H1299 cells in 2% PFA and stained with 0.5% crystal violet. I have air dried them...

08 March 2016 770 5 View

Protocol for sample preparation for zymography from cell line?

Can someone give me a detailed protocol of sample preparation from cell line for zymography study. Addition of protease inhibitors, speed vac to concentrate the samples, bradford for estimation...

15 July 2014 8,389 5 View