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I would like to know the exact protocol to induce chemical hypoxia in A549 cells. There are some variations that I have noticed in the method in various papers which are confusing. Do we starve...
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I normally starve cells for 24 hours in serum free media and then add my drug in serum media, scratch and add this treatment media and follow the healing. In case of HaCat I starved the cell in 1%...
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