How to separate D-Arabinose and L-Arabinose using HPLC?

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Isam Eldin Hussein Elgailani

Please look at the following below links which may help you in your analysis:

https://pubag.nal.usda.gov/pubag/downloadPDF.xhtml?id=54201&content=PDF

http://agris.fao.org/agris-search/search.do?recordID=KR2008003732

Thanks

Bruce Neagle

It depends on your sample matrix and expected concentration. However, the method has to be chiral (as the retention method).

Sara Thamer Hadi

following

You can review this website. It is managed by Bill Letter the foremost chiral chemist that I know. In any case I suspect it will be an amino column in the HILIC mode in order just to separate the sugars from the other crap.

https://hplctips.blogspot.com/

And review this website;

https://www.phenomenex.com/Products/HPLCDetail/lux?gclid=Cj0KCQjwwODlBRDuARIsAMy_28X3GZRAPI98J4CEJPhab-TnMS5bL90b2BbUBlV0kBL_pXwOBmZam_IaAj7bEALw_wcB