Dear Sir,

Levamisole. Interest to the drug decreased. There are side effects.

http://en.wikipedia.org/wiki/Levamisole

https://ru.wikipedia.org/wiki/Левамизол

Immunostimulants. Widely used in experimental studies and clinic for the treatment of immunodeficiency states:

 http://ru.wikipedia.org/wiki/Полиоксидоний

http://petrovax.com/medication/catalog/polyoxydonium/

https://ru.wikipedia.org/wiki/Т-активин

http://www.reference.md/files/C036/mC036191.html

http://link.springer.com/article/10.1007/BF00830265

https://ru.wikipedia.org/wiki/Тимоген#.D0.9A.D0.BB.D0.B8.D0.BD.D0.B8.D1.87.D0.B5.D1.81.D0.BA.D0.BE.D0.B5_.D0.BF.D1.80.D0.B8.D0.BC.D0.B5.D0.BD.D0.B5.D0.BD.D0.B8.D0.B5

http://ru.wikipedia.org/wiki/Имунофан

https://ru.wikipedia.org/wiki/Миелопид

Leflunomide (brand names: Arabloc, Arava, Lunava, Repso) is an immunosuppressive disease-modifying antirheumatic drug (DMARD), used in active moderate to severe rheumatoid arthritis and psoriatic arthritis. It is a pyrimidine synthesis inhibitor.

http://en.wikipedia.org/wiki/Leflunomide

http://www.rlsnet.ru/mnn_index_id_2819.htm

There are other immunosuppressants. I do not quite clear the purpose of your research.

Dear Tiwari,

Look at first instance to the toxicity effects of interleukine 1, TNF-alpha and dexamethasone. Other immunoregulators can be examined too.

This are clear activators and stimulators.

Depending on new drugs in between effects can be expected.

Kind regards

willem

Read my articles and books in the RG - where answers to many questions. Unfortunately everything is written in Russian, but there is a summary in English.

What is the impact of immunostimulatory and immunosuppressive drugs on the following parameters?

1 Parameters there is much more. But of these parameters can be estimated ...

2 The question is very complex and it can not be answered briefly.

3 Changes to the parameters is different for many immunostimulatory and immunosuppressive drugs.

Many immunosuppressive drugs act like toxic substances (toxicants). Effect in immunosuppressive drugs have a "more selective" than the toxicants. There are many similarities. A number of toxicants also act selectively.

Dear Sir,

I would be grateful to you if you could share those documents to the series of this questions. I would like to know about impact of Levamisole (stimulatory) and leflunomide (suppressive) on mice in terms of above mentioned parameters (Body weight of animals (in vitro study) and Thymus and spleen weight, DTH and HA titer.

Similarly what will be their impact in in vivo studies.

Dear Sir,

Levamisole. Interest to the drug decreased. There are side effects.

http://en.wikipedia.org/wiki/Levamisole

https://ru.wikipedia.org/wiki/Левамизол

Immunostimulants. Widely used in experimental studies and clinic for the treatment of immunodeficiency states:

 http://ru.wikipedia.org/wiki/Полиоксидоний

http://petrovax.com/medication/catalog/polyoxydonium/

https://ru.wikipedia.org/wiki/Т-активин

http://www.reference.md/files/C036/mC036191.html

http://link.springer.com/article/10.1007/BF00830265

https://ru.wikipedia.org/wiki/Тимоген#.D0.9A.D0.BB.D0.B8.D0.BD.D0.B8.D1.87.D0.B5.D1.81.D0.BA.D0.BE.D0.B5_.D0.BF.D1.80.D0.B8.D0.BC.D0.B5.D0.BD.D0.B5.D0.BD.D0.B8.D0.B5

http://ru.wikipedia.org/wiki/Имунофан

https://ru.wikipedia.org/wiki/Миелопид

Leflunomide (brand names: Arabloc, Arava, Lunava, Repso) is an immunosuppressive disease-modifying antirheumatic drug (DMARD), used in active moderate to severe rheumatoid arthritis and psoriatic arthritis. It is a pyrimidine synthesis inhibitor.

http://en.wikipedia.org/wiki/Leflunomide

http://www.rlsnet.ru/mnn_index_id_2819.htm

There are other immunosuppressants. I do not quite clear the purpose of your research.

Dear Sir thank you so much for your valuable links.

I just wanted to know that these compounds (levamisole and leflunomide) if used as controls for in vitro or in vivo immunomodulatory studies in cell lines or animals;

What will be there effect on the above mentioned parameters.

I would like to assess the immnunomodulatory potential of phytochemical fractions and wanted to use these compounds (levamisole and leflunomide) as control.

Waiting for your kind prompt reply.

Dear Bhavesh Tiwari, 

If I understand you correctly you are interested levamisole and leflunomide?

Or you can use other immunostimulants and immunosuppressants?

You can use levamisole as an immunostimulant, and leflunomide as an immunosuppressant (I gave links to the sources of literature).

I think that for the implementation of your application, you can use these drugs, although I would take the other (see the links to the sources of literature that I have outlined above).

Dear Sir, Thank you so much for your kind and prompt reply,

If I am using levamisole and leflunomide in my animal studies

what will be their effect on

1. Body weight of animals: Whether they will increase it or decrease

2. Thymus and spleen weight: Whether they will increase it or decrease

3. Blood cells (WBC's); Whether it will increase or decrease

4. Delayed Type hypersensitivity and Haemagglutination titre: Whether they will increase or decrease the response

5. Plaque forming cells: Whether they will increase it or decrease

If I am using levamisole and leflunomide in cell lines

what will be their effect on

1. NO production in macrophages: Whether they will increase it or decrease

2. Oxidative burst; Whether they will increase it or decrease

Summarized as Drug effects of a mineral complex solution - increases or decreases the expression of blood cells WBC sub populations in treated, determined by use of specific antibodies and flow cytometry. This immunostimulatory effect could supports use of the drug as an alternative to antimicrobials for improving productivity in the animal industry.

Byung Woo Yoo, et all (2001), Immunostimulatory effects of an anionic alkali mineral complex solution (Barodon®) on porcine lymphocytes.

Dear Bhavesh Tiwari,

answers to many of your questions are obvious. There are a lot of literature data.

Something I do not know. Explore!

The correct answers will give you a pilot study.

You want me you predicted the results of experiments?

I can make suggestions...

I wish you success in your experimental studies!