Hi all,
We are trying to find potential TF regulators of the gene we are working on. We plan to use Y1H assay to do a large scale screening. But we don't know how to determine the region of the promoter we can use as a bait, because the putative cis-elements are scattering on the promoter region. Indeed, there is a region enriched with several cis-elements. We decide to test this region first. Anyone can tell how long fragment is suitable for a Y1H library screening? I was told that a piece should no more than 200 bp, otherwise, we may get false positive clones. However, I was thinking even false positive signals disturb, as long as it is not too many, it is acceptable for our purpose. Can anyone give suggestions?
Thanks for your kind help in advance.
Chen
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