Chen Chen

12 Questions 18 Answers 0 Followers

Questions related from Chen Chen

How to select promoter region for a yeast-one-hybrid assay to identify putative trans-factors?

Hi all, We are trying to find potential TF regulators of the gene we are working on. We plan to use Y1H assay to do a large scale screening. But we don't know how to determine the region of the...

10 July 2018 3,543 0 View

How to efficiently detect potential interactions between multiple DNA cis-elements and multiple proteins?

Hi guys, I have a bunch of short DNA sequences (about 300) can potentially interact with ARF transcription factors according to cis-elements analysis. There are 25 ARFs encoded by the genome. Is...

26 December 2017 5,797 4 View

How dose the methylation inhibitor 5-aza-2'-deoxycytidine function in plant?

5-aza-2'-deoxycytidine is a widely used meythylation inhibitor, which inactivates the DNA methyltransferase of animals. This chemical also works for plants. However, in terms of the methylation...

28 June 2017 5,406 3 View

What's the function of genic CHG methylation?

Hi all, I found two groups of genes showed differential CHG methylation in gene body regions, I am wondering the potential biological significance of The difference. Can anyone give some...

13 May 2017 3,392 0 View

Dose yeast survive on -Met medium?

I am doing transcriptional activation activity assay in yeast. To confirm if the two genes I am working on are required for the transcriptional activity, I used pBridge to express my targets. I...

08 May 2017 8,987 4 View

How to determine a pseudogene?

Hi all, I searched my genes against pseudogene database (http://www.pseudogene.org/) and found some of them are considered as pseudogenes. However, according the the annotation database, most of...

12 June 2016 9,223 3 View

Is yeast strain AH109 good for X-gal assay?

We encountered a problem for testing protein protein interaction by Y2H assay. The AH109 transformants carrying empty pGBK and pGAD vectors can still survive on triple SD medium (-Leu-Trp-His)...

10 May 2016 3,535 2 View

Does the hull size determine grain (brown seed) size in monocots?

I am curious about the relationship between the hull size and grain size. Usually big hulls give big grains, which suggests that hull size is a determinator of grain size. My question would be the...

01 November 2015 5,312 1 View

How if I missed several nucleotides in the promoter of a fused gene?

I made a fused gene using gene 1 promoter to drive gene 2 expression. Because 3' of the gene 1 promoter is GC only, it was difficult to design primer for promoter amplification. To avoid this...

27 October 2015 9,376 3 View

Does maize ubiquitin promoter work for Arabidopsis?

Hi all, I am working on a rice gene and trying to see if the gene can rescue Abrabidopsis mutant. I used maize ubiquitin promoter to drive gene over-expression in rice. I am not sure if the same...

27 October 2015 6,412 3 View

Who has a cytokinin reporter system (TCS:GUS/GFP)?

Hi, Does anyone have or know where to get a cytokinin reporter system using a TCS promoter driving GFP or GUS expression? It will be really appreciated if you would like to share such...

03 November 2014 441 1 View

How can I distinguish plant senescence and PCD and which could be the marker genes for senescence and PCD in rice?

I am working on a rice mutant which showed leaf yellowing phenotype. Initially, very tiny lesion mimic spots appeared randomly on the old leaves, then the spots expand and finally, the leaves...

13 July 2014 471 7 View