How to select genes for MLST?

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Formation of a target organizational and functional model for data management in space activities?

Formation of a target organizational and functional model for data management in space activities

10 July 2024 4,498 0 View

What connection can there be between politics and collective intelligence?

I am interested in reflecting on the political dimension of collective intelligence.

13 April 2024 2,811 18 View

How can the concentration of colloidal particles in an aqueous solution be determined?

How can the concentration of colloidal particles in an aqueous solution be determined? Is it possible to apply the photo-colorimetric method for this?

11 October 2023 1,563 21 View

Where can I find data about viscosity dependence on concentration in methanol–poly(vinyl acetate) solution?

Where can I find data about viscosity dependence on concentration in methanol–poly(vinyl acetate) solution? What value of saturation concentration and diffusion coefficient for the solved polymer...

11 April 2023 3,414 2 View

Is there the state support for medical insurance of radioisotope diagnostics in your country?

Radioisotope diagnostics of many diseases is currently available in many countries. But this is often an expensive procedure. Does your country provide state support for medical insurance for...

01 April 2023 3,895 7 View

I'm looking for a good review article/book chapter about parrallel methods based on simulated annealing. Any recommendations?

I am currently studying the application of simulated annealing techniques to optimization problems. In particular, I am interested in applying the optimization method to the study of Ising models....

21 November 2022 2,663 1 View

Amplification in regenerative amplifier by cw pumping?

Hello. In real moment I'm designing the CPA system for my Ti:Sa laser. I have only cw diode module for pumping (10 W). How can I evaluate amplification opportunity in 'regen'. I want to gain from...

18 September 2022 5,450 1 View

Why is there increase of electrode atomic forces when applying bias voltage in TranSiesta?

Dear colleagues, I am trying to calculate I-V characteristics for typical (Left electrode) - (Device) – (Right electrode) system. Besides the well-known problem of difficult electronic...

27 March 2022 8,532 11 View

How to block an IR beam in vacuum?

Hallo, I've a HHG setup, but the IR filter is broken. This means that the entire IR intensity hits the CCD camera and would destroy it. Because a replacement filter needs a few weeks to arrive,...

27 February 2022 5,470 1 View

How to determine the fraction of DNA from lymphocytes in the blood?

I'm thinking about how to detect in circulating free DNA fraction that comes from white blood cells. Now my idea is to use T cell receptor excision circle (TREC) and kappa-deleting recombination...

01 June 2021 5,918 0 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Who of all the Global Scientific community will help me Prof. Dr. Yoshida make way for TPEOM, MEC ~EMC to return the atmospheric gases to the norma ?

TEP presentation caption (The Environmental Project) Re: Why should Washington’s DC, or any country government point of location think of as nowadays of as to being 'tomorrow as to come! if it...

03 August 2024 2,484 1 View

How is the bacterial genome's high protein count verified as genuine despite 800+ contigs and good metrics (98.55%completeness, 0.68% contamination)?

Given that the bacterial genome has over 800 contigs, but its quality metrics are good, with a completeness of 98.55% and a contamination of 0.68% as assessed by CheckM, what specific validation...

01 August 2024 1,514 1 View

Seeking Software Recommendations for SELEX NGS Data Analysis?

I am looking for software to help analyze SELEX NGS data, including alignment, sequence enrichment, and other related tasks. Can anyone recommend suitable tools or software? Best wishes, Waleed

30 July 2024 1,061 5 View

Recovery Viurses from bacteria genome?

Hello everyone, I am currently looking for tools to recovery viral genomes from bacterial genomes, not metagenomes. However, I have only found tools that are designed for retrieving and studying...

28 July 2024 8,953 1 View

Could you try using PeptiCloud and see if it's a useful tool for biology research?

PeptiCloud (www.pepticloud.com) is a bioinformatic platform that allows researchers to organize and share their data for their projects as well as collaborate with others in one place. Through...

28 July 2024 4,762 2 View

Should the amount of DNA input used for ChIP-seq library preparation be matched between the control and experimental groups?

Hi all. As a beginner in ChIP-seq experiments, I hope you understand that the following questions might be somewhat basic. I am planning to perform ChIP-seq or MeDIP-seq analysis to investigate...

28 July 2024 6,938 1 View

Why my negative control siRNA is decreasing the target gene's expression?

Hi Everyone, I'm using an siRNA kit to knock down a target gene. The kit guarantees that the negative control doesn't target any sequence in mouse genome, and when I use BLAST I don't find any...

23 July 2024 2,673 6 View

How to use NCBI datasets ?

I have been trying to extract genome from NCBI using their dataset tool, however some examples seem not to work : ./datasets download genome taxon "Homo Sapiens" --annotated --assembly-level...

20 July 2024 1,339 2 View