Axon damage is one of the hallmarks in Multiple Sclerosis. My question is if I want to see axonal damage in mice model then what types of techniques and reagents I have to use?
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Kaushiki Biswas
You can use some neuronal markers or silver stain to label the neurons in tissue sections/slices and can quantify the reduction of neuronal staining. That's one technique.
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Sebastián Dupraz
Hi,
Depending on the onset of the degeneration in your model, you can try to express GFP or axon-specific membrane targeted GFP by infection/electroporation of the areas you want to study. Then, once the disease has developed you'll have a good signal to follow axons and quantify their density between control and deseased tissue.
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