How anxiety can be separated from curiosity in open field test for mice?

It is seen that in open field test mice do rearing more suggesting they are more exploratory or curious. Now it is considered one of the markers of anxiety like behaviour. But it is curiosity I...

01 February 2019 1,157 2 View

Elevated plus maze VS. open field anxiety test?

For the detection of anxiety of mouse which test is more suitable and why? Thanks, MS

01 February 2019 2,780 1 View

Meaning of increased rearing and grooming?

I have found my mice showed more rearing and grooming but my question is why they do that more and what does it indicates? Thanks MS

01 February 2019 4,927 7 View

Hyperactivity in mouse?

How the hyperactivity of mouse can be measured and how we can understand that these mice are hyperactive? Thanks MS

01 February 2019 1,573 1 View

When is the best time to do mice behavior test?

Mice are naturally nocturnal but most of the lab behavioral tests are conducted in the day time. Is this confounding? Therefore when is the best time to do behavioral test of mice/rodents? Thanks...

31 December 2018 509 4 View

How many behavioral tests can be conducted in parallel in mice?

For some reasons most of the time multiple behavioral tests such as open field, rotarod, walking beam, and novel object recognition need to conduct in parallel. However, there may be time issue...

31 December 2018 8,055 3 View

Why oligodendrocytes behave differently in different parts of the CNS?

It is observed that oligodendrocytes functions differs based on its position in CNS, why?

31 December 2018 3,358 2 View

Which is better test to assess anxiety in mouse?

Dears, To test anxiety and locomotion commonly "Open filed" and "Elevated-plus maze" apparatus are used. However, for certain reasons multiple instruments is not possible to use in same study. To...

31 December 2018 4,901 4 View

Activator of B or T cells

31 December 2016 2,492 0 View

Which neurofilament will be appropriate to examine axonal damage in animal model?

We know there are three forms of neurofilaments and release of neurofilaments after axonal damage are prevalent in Multiple Sclerosis patients. My question is in animal model of MS what type of...

02 March 2016 2,743 6 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

No title

03 March 2021 5,360 2 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View