Is it possible to isolate RNA from flash frozen whole blood samples?

I understand that most RNA isolation kits, like QIAamp minikit cannot be used for isolating RNA from frozen samples. Are there any kits which may be used? what about manual methos with trizol

09 October 2017 5,325 4 View

What could cause mouse skeletal muscle to become 'dry' and 'less fleshy'?

10 November 2016 6,661 13 View

Surrogate marker for insulin resistance?

I have frozen mice livers, pancreas and skeletal muscle. The blood sample obtained at the time of sacrificing the animals was just enough for complete lipid profile. i need to get an idea of...

07 August 2016 2,548 7 View

Has anyone tried Qiagen one step real time PCR kit? Why is it so much more expensive than say fermenats kit?

Has anyone tried Qiagen one step real time PCR kit? Why is it so much more expensive than say fermenats kit? Does it have some extra features? more reliability?

04 May 2016 451 1 View

16 S ribosomal RNA equivalent for viruses in NGS?

03 April 2016 3,493 6 View

Has anybody tried western blotting related buffers antibodies etc from Sigma?

Has anybody tried western blotting related buffers, antibodies etc from Sigma? How do they compare with products from abcam?

31 December 2015 6,491 6 View

Will real time PCR primers designed for one strain of mouse work on another strain?

09 October 2015 7,067 7 View

Could an intraperitoneal drug affect epidydmal fat pad directly in mouse?

01 January 1970 4,357 0 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

How to manually calculate p value in ANOVA? what is the equation or formula for it?

How to manually calculate p value in ANOVA? what is the equation or formula for it?

03 March 2021 4,928 5 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature)?

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...

03 March 2021 2,870 3 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View