How to report results of split plot repeated measures ANOVA?

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Is it possible to isolate RNA from flash frozen whole blood samples?

I understand that most RNA isolation kits, like QIAamp minikit cannot be used for isolating RNA from frozen samples. Are there any kits which may be used? what about manual methos with trizol

09 October 2017 5,430 4 View

What could cause mouse skeletal muscle to become 'dry' and 'less fleshy'?

When we dissected our mice after treatment we found that the skeletal muscle in the treatment group were 'dry' and 'less fleshy' as compared to placebo.The mice were otherwise active and healthy...

10 November 2016 6,785 13 View

Surrogate marker for insulin resistance?

I have frozen mice livers, pancreas and skeletal muscle. The blood sample obtained at the time of sacrificing the animals was just enough for complete lipid profile. i need to get an idea of...

07 August 2016 2,657 7 View

Has anyone tried Qiagen one step real time PCR kit? Why is it so much more expensive than say fermenats kit?

Has anyone tried Qiagen one step real time PCR kit? Why is it so much more expensive than say fermenats kit? Does it have some extra features? more reliability?

04 May 2016 563 1 View

16 S ribosomal RNA equivalent for viruses in NGS?

Hello, I wanted to know if there was a general test/s by which we could determine if there is a viral infection without having to specify which virus. Like with sequencing 16 s rRNA by NGS we can...

03 April 2016 3,707 6 View

Has anybody tried western blotting related buffers antibodies etc from Sigma?

Has anybody tried western blotting related buffers, antibodies etc from Sigma? How do they compare with products from abcam?

31 December 2015 6,597 6 View

Will real time PCR primers designed for one strain of mouse work on another strain?

I found primer sequences for checking genetic expression of leptin in Aston strain mice. I was wondering if they would work on tissues harvested from swiss webster mice?

09 October 2015 7,178 7 View

Could an intraperitoneal drug affect epidydmal fat pad directly in mouse?

If a drug is administered intraperitoneally in a mouse could it have access to the subcutaneus fat pad directly? instead of first being absorbed into mesenteric vessels--> portal vein-->...

01 January 1970 4,467 0 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Handling Missing Data and Building a Predictive Model with Incomplete Information ?

I am developing a predictive model for a water supply network that involves 20 influencing points. However, I only have historical data for 10 out of these 20 points. I would like to know how to...

10 August 2024 4,005 2 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

How are iso-frequency contours plotted?

Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...

07 August 2024 1,937 1 View

How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.

07 August 2024 5,307 1 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Rainer Duesing

Please have a look at Andy Field - Discovering Statistics Using SPSS (also for R and SAS). Throughout his chapters he shows how to interpret and report the findings. Please see links below.

https://uk.sagepub.com/en-gb/eur/discovering-statistics-using-ibm-spss-statistics/book238032#description

http://www.statisticshell.com/html/apf.html

Tania Ahmed Shakoori

Thank you