Is it possible to isolate RNA from flash frozen whole blood samples?

I understand that most RNA isolation kits, like QIAamp minikit cannot be used for isolating RNA from frozen samples. Are there any kits which may be used? what about manual methos with trizol

09 October 2017 5,325 4 View

What could cause mouse skeletal muscle to become 'dry' and 'less fleshy'?

10 November 2016 6,661 13 View

Surrogate marker for insulin resistance?

I have frozen mice livers, pancreas and skeletal muscle. The blood sample obtained at the time of sacrificing the animals was just enough for complete lipid profile. i need to get an idea of...

07 August 2016 2,548 7 View

16 S ribosomal RNA equivalent for viruses in NGS?

03 April 2016 3,493 6 View

How to report results of split plot repeated measures ANOVA?

Can anyone recommend a paper which used split plot ANOVA for their data analysis. i want to see how it is reported

01 February 2016 7,884 2 View

Has anybody tried western blotting related buffers antibodies etc from Sigma?

Has anybody tried western blotting related buffers, antibodies etc from Sigma? How do they compare with products from abcam?

31 December 2015 6,491 6 View

Will real time PCR primers designed for one strain of mouse work on another strain?

09 October 2015 7,067 7 View

Could an intraperitoneal drug affect epidydmal fat pad directly in mouse?

01 January 1970 4,357 0 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View