Hi,

I have found two SNP (Alanine to serine & threonine) at different positions in my desired gene (Sanger sequencing), and then I have built a protein 3D structure by using I-Tasser and swiss-model servers. When I replaced SNPs (at a particular point) with mutated one, it's making a hydrophobic bond there and protein showing the increase in negative delta G (making sense that protein getting more stable). My protein is a glycosylated protein and now I want to replace these desired mutated positions with glycosylated amino-acid But I don't know how to replace or add a glycan or phosphate group at my desired positions. Kindly, Please tell me the software or procedures to replace an amino-acid with glycosylated/phosphorylated amino-acid.

Thank you in advance.