SeqClean software

06 July 2014 2,626 1 View

Why should we put leaves in the dark after agrobacterium treatment?

Why is a dark incubation period is important for agrobacterium-mediated transformation?

01 February 2014 7,745 4 View

Any advice on plasmid extraction?

We have a plasmid construct that either cannot be extracted by miniprep methods, or if extracted, only in tiny amounts. The backbone of this construct is PUC19. Any suggestions to overcome this...

11 December 2013 9,282 11 View

Why should we put leaves in darkness after shooting by biolistic?

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11 December 2013 9,201 3 View

Any advice on albinism after shooting by biolistic?

After we shoot our construct using biolistic equipment, the plants grow on the antibiotic medium. However, after 1 month, they become white. We use lettuce flanks for tobacco chloroplast...

11 December 2013 2,430 1 View

Kozak consensus sequence - can anyone help?

I want to express a human gene in the tobacco plant. My gene does not have any kozak consensus sequence or even kozak like sequence upstream of the ATG. Do I need to insert it in my construction...

09 October 2013 4,963 2 View

Double digestion problem

In order to do cloning, we had done double digestion of plasmid with SphI and KpnI restriction enzymes; but after electrophoresis, we did not see any band on the gel. The enzymes are produced by...

09 October 2013 930 9 View

How to optimize codon usage?

I want to optimize codon usage of a human gene for expression in a plant (Nicotiana tabacum). How can to do it? Is codon harmonization necessary or not?

08 September 2013 5,077 9 View

Bright green in Wild type vessel elements

I want to expression GFP in lettuce. I checked wild type plant by fluorsecent microscope. I saw bright green in vessel elements (attached file). what may be the reason? What should I do...

07 August 2013 4,651 8 View

What ts different between bacteriological agar and plant agar?

I want to use Murashige and Skoog (MS) medium culture. What is the difference between bacteriological agar and plant agar?

07 August 2013 6,536 7 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View