I'm going to remove poly-A tail of an EST library. Could anyone help me to how can I do this?
Dear Soren
Thanks a lot
I want to work with SeqClean software, but I don't know how do it. Could anyone help me to how install and work with this software?
06 July 2014 2,789 1 View
Why is a dark incubation period is important for agrobacterium-mediated transformation?
01 February 2014 7,850 4 View
We have a plasmid construct that either cannot be extracted by miniprep methods, or if extracted, only in tiny amounts. The backbone of this construct is PUC19. Any suggestions to overcome this...
11 December 2013 9,508 11 View
.
11 December 2013 9,355 3 View
After we shoot our construct using biolistic equipment, the plants grow on the antibiotic medium. However, after 1 month, they become white. We use lettuce flanks for tobacco chloroplast...
11 December 2013 2,593 1 View
I want to express a human gene in the tobacco plant. My gene does not have any kozak consensus sequence or even kozak like sequence upstream of the ATG. Do I need to insert it in my construction...
09 October 2013 5,136 2 View
In order to do cloning, we had done double digestion of plasmid with SphI and KpnI restriction enzymes; but after electrophoresis, we did not see any band on the gel. The enzymes are produced by...
09 October 2013 1,291 9 View
I want to optimize codon usage of a human gene for expression in a plant (Nicotiana tabacum). How can to do it? Is codon harmonization necessary or not?
08 September 2013 5,415 9 View
I want to expression GFP in lettuce. I checked wild type plant by fluorsecent microscope. I saw bright green in vessel elements (attached file). what may be the reason? What should I do...
07 August 2013 4,771 8 View
I want to use Murashige and Skoog (MS) medium culture. What is the difference between bacteriological agar and plant agar?
07 August 2013 6,747 7 View
I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.
11 August 2024 9,101 4 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...
10 August 2024 7,180 0 View
I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...
10 August 2024 7,429 2 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View