Any advice on albinism after shooting by biolistic?

12 December 2013 1 3K Report

After we shoot our construct using biolistic equipment, the plants grow on the antibiotic medium. However, after 1 month, they become white. We use lettuce flanks for tobacco chloroplast transformation. Any suggestions?

Katayoun Zamani

Dear Mostafa

There are two reasons for plant whitening if you confirm the presence of the transgene in plants.

1- The transgene is silenced. I have such experience with Arabidopsis and 35S promoter and after 3 to 4 weeks I couldn't see transgene expression.

2- The concentration of selective antibiotic is very high; for saving your plants,you should decrease antibiotic concentration in selection medium.

Badges
Science topic

More Mostafa Modarresi's questions See All

SeqClean software

I want to work with SeqClean software, but I don't know how do it. Could anyone help me to how install and work with this software?

06 July 2014 2,789 1 View

How to remove Poly-a tail?

I'm going to remove poly-A tail of an EST library. Could anyone help me to how can I do this?

06 July 2014 3,829 1 View

Why should we put leaves in the dark after agrobacterium treatment?

Why is a dark incubation period is important for agrobacterium-mediated transformation?

01 February 2014 7,850 4 View

Any advice on plasmid extraction?

We have a plasmid construct that either cannot be extracted by miniprep methods, or if extracted, only in tiny amounts. The backbone of this construct is PUC19. Any suggestions to overcome this...

11 December 2013 9,508 11 View

Why should we put leaves in darkness after shooting by biolistic?

11 December 2013 9,355 3 View

Kozak consensus sequence - can anyone help?

I want to express a human gene in the tobacco plant. My gene does not have any kozak consensus sequence or even kozak like sequence upstream of the ATG. Do I need to insert it in my construction...

09 October 2013 5,136 2 View

Double digestion problem

In order to do cloning, we had done double digestion of plasmid with SphI and KpnI restriction enzymes; but after electrophoresis, we did not see any band on the gel. The enzymes are produced by...

09 October 2013 1,291 9 View

How to optimize codon usage?

I want to optimize codon usage of a human gene for expression in a plant (Nicotiana tabacum). How can to do it? Is codon harmonization necessary or not?

08 September 2013 5,415 9 View

Bright green in Wild type vessel elements

I want to expression GFP in lettuce. I checked wild type plant by fluorsecent microscope. I saw bright green in vessel elements (attached file). what may be the reason? What should I do...

07 August 2013 4,771 8 View

What ts different between bacteriological agar and plant agar?

I want to use Murashige and Skoog (MS) medium culture. What is the difference between bacteriological agar and plant agar?

07 August 2013 6,747 7 View

Geotechnical Engineering (Proceedings of the ICE) time review?

Hello everyone, I recently submitted an article to Geotechnical Engineering (Proceedings of the ICE), and the current status has been listed as "EiC Pre-assessment: Ready" for the past 20 days. I...

10 August 2024 6,493 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

How can we now if the promotor in the vector is well?

Hi, everyone. I want to transfer two genes in to the pseudomonas bacteria that isolated from the soil. The bacteria that I want to use for cloning haven't been identified and not be sequenced,...

02 August 2024 3,987 1 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View