15 Questions 20 Answers 0 Followers
Questions related from Mostafa Modarresi
I want to work with SeqClean software, but I don't know how do it. Could anyone help me to how install and work with this software?
07 July 2014 2,754 1 View
I'm going to remove poly-A tail of an EST library. Could anyone help me to how can I do this?
07 July 2014 3,728 1 View
Why is a dark incubation period is important for agrobacterium-mediated transformation?
02 February 2014 7,805 4 View
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12 December 2013 9,317 3 View
We have a plasmid construct that either cannot be extracted by miniprep methods, or if extracted, only in tiny amounts. The backbone of this construct is PUC19. Any suggestions to overcome this...
12 December 2013 9,461 11 View
After we shoot our construct using biolistic equipment, the plants grow on the antibiotic medium. However, after 1 month, they become white. We use lettuce flanks for tobacco chloroplast...
12 December 2013 2,550 1 View
In order to do cloning, we had done double digestion of plasmid with SphI and KpnI restriction enzymes; but after electrophoresis, we did not see any band on the gel. The enzymes are produced by...
10 October 2013 1,214 9 View
I want to express a human gene in the tobacco plant. My gene does not have any kozak consensus sequence or even kozak like sequence upstream of the ATG. Do I need to insert it in my construction...
10 October 2013 5,089 2 View
I want to optimize codon usage of a human gene for expression in a plant (Nicotiana tabacum). How can to do it? Is codon harmonization necessary or not?
09 September 2013 5,299 9 View
I want to expression GFP in lettuce. I checked wild type plant by fluorsecent microscope. I saw bright green in vessel elements (attached file). what may be the reason? What should I do...
08 August 2013 4,740 8 View
I want to use Murashige and Skoog (MS) medium culture. What is the difference between bacteriological agar and plant agar?
08 August 2013 6,665 7 View
For AFLP we must do Restriction-Ligation with two Adapter and restriction enzymes. Maybe the DNA is cut with same restriction enzyme, therefore PCR is performed with one primer. How we can...
07 July 2013 2,026 2 View
I have one tobacco plant that infected by turnip mosaic virus (TuMV). I need a prptocol for isolation TuMV from plant.
07 July 2013 8,910 4 View
We want to use vacuum infiltration for transient expression in tobacco and lettuce. We wonder if anyone could suggest us a good protocol?
07 July 2013 5,668 10 View
If we have a systemic virus vector with coat protein, can we use agrobacterium for nonsystemic transformation of plants? Is this magnifection method?
06 June 2013 2,674 2 View
