How to remove Poly-a tail?

I'm going to remove poly-A tail of an EST library. Could anyone help me to how can I do this?

06 July 2014 3,625 1 View

Why should we put leaves in the dark after agrobacterium treatment?

Why is a dark incubation period is important for agrobacterium-mediated transformation?

01 February 2014 7,745 4 View

Any advice on plasmid extraction?

We have a plasmid construct that either cannot be extracted by miniprep methods, or if extracted, only in tiny amounts. The backbone of this construct is PUC19. Any suggestions to overcome this...

11 December 2013 9,282 11 View

Why should we put leaves in darkness after shooting by biolistic?

.

11 December 2013 9,201 3 View

Any advice on albinism after shooting by biolistic?

After we shoot our construct using biolistic equipment, the plants grow on the antibiotic medium. However, after 1 month, they become white. We use lettuce flanks for tobacco chloroplast...

11 December 2013 2,430 1 View

Kozak consensus sequence - can anyone help?

I want to express a human gene in the tobacco plant. My gene does not have any kozak consensus sequence or even kozak like sequence upstream of the ATG. Do I need to insert it in my construction...

09 October 2013 4,963 2 View

Double digestion problem

In order to do cloning, we had done double digestion of plasmid with SphI and KpnI restriction enzymes; but after electrophoresis, we did not see any band on the gel. The enzymes are produced by...

09 October 2013 930 9 View

How to optimize codon usage?

I want to optimize codon usage of a human gene for expression in a plant (Nicotiana tabacum). How can to do it? Is codon harmonization necessary or not?

08 September 2013 5,077 9 View

Bright green in Wild type vessel elements

I want to expression GFP in lettuce. I checked wild type plant by fluorsecent microscope. I saw bright green in vessel elements (attached file). what may be the reason? What should I do...

07 August 2013 4,651 8 View

What ts different between bacteriological agar and plant agar?

I want to use Murashige and Skoog (MS) medium culture. What is the difference between bacteriological agar and plant agar?

07 August 2013 6,536 7 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View