I am trying to do His pull-down to search for interacting proteins of my protein, which is tagged with Myc His at the C terminal end. I have cloned my protein in the pCDNA 3.1 vector and transfecting the construct to HEK 293T cells followed by pull down with Ni NTA columns.
However I am getting lots of non specific bands in the vector transfected cells themselves. Do you have any suggestions as to how to remedy this?