Goutam Kumar Tanti

7 Questions 11 Answers 0 Followers

Questions related from Goutam Kumar Tanti

Does fixation affect PTM of antigen?

Does anybody have any idea whether fixation protocol for IHC affects the post translational modification of antigens? Thank you in advance!

23 October 2017 3,777 2 View

Will an inter-species fusion antibody be as effective as a wild type?

If we fuse conserved region of antibody from mouse to a variable region from rat will the fusion antibody work with similar efficiency? Or is there any other method to murinise the sequence of...

03 September 2014 6,930 5 View

What is the maximum molecular weight shift after phosphorylation?

During my Masters my teacher told me there is around 20 kDa molecular weight shift after excessive phosphorylation of a protein.. Problem is I forgot the name of the protein. Does anybody remember...

22 August 2014 1,576 1 View

What is the best way to murinize a sequence?

We raised monoclonal antibody against a human protein in rat. From that hybridoma clone I can get the sequence of the antibody. I want to clone that antibody and express it in mouse. I need to...

31 July 2014 4,761 3 View

Lentiviral preparation?

I tried preparing lentiviral particles with pLKO.1 system with psPAX2 packaging plasmid and pMD2.G envelope plasmid in ratio of 1: 0.75: 0.250. I tried twice with Lipofectamine LTX reagent in...

06 January 2014 7,005 3 View

What is the criteria for co-localization?

I have read some papers where the authors showed that parcentage of cells harboring parkin in mitochondria (e.g. "Parkin is recruited selectively to impaired mitochondria and promotes their...

05 July 2013 2,867 1 View

How to remove non-specific bands from His pull down?

I am trying to do His pull-down to search for interacting proteins of my protein, which is tagged with Myc His at the C terminal end. I have cloned my protein in the pCDNA 3.1 vector and...

15 April 2013 7,259 4 View