I am trying to extract cancer cells from human gastric cancer tissue. I noticed that, after digesting the tissue, I have a lot of lipids present in my cell suspension. When I try to pellet down the cells (after I add serum to stop the digestion), the cells will still float and I cannot pellet them. I tried to remove the tissue fat before digestion but I cannot get rid of all and I still end up losing cells. Does anyone knows a treatment that I could use to remove the lipids without affecting the collection and viability of the cancer cells ?
Thanks
You can try collagenase digestion for 2 hours. It is very effective than other methods of digestion for adipose tissue fractionation.
Dear Mathieu
I agree with Muruganandan ref using collegians digestion. However, you may have include trypsin to get maximum yield. Please visit
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3787097/
I have no idea why the cancer cells could float with lipids? What method you used for digestion? two answers mentioned about collagenase method which is usrualy used for digestion adipose tissue that will not help you in your case. I suggestion you can peppet well your diagestion mix that let cancer cell seperate from lipids, and spin dowm the mixture at 200g, for 10 min, it may be help you. So let me know if it is work.
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