I understand that wound healing requires cell migration and cell proliferation. I would like to know which cell receptors are activated during the cell migration part of the wound healing assay? Are they similar to those activated during chemotaxis?
Thats tricky. When we analyse by chemotaxis, usually focus is on certain specific cytokines (for specific cell types), so respective receptors could be involved. In case of wound healing assay in the presence of FCS containing medium all present cytokines (may be EGF, FGF, VEGF), which can induce cell polarization / alter actin dynamics should contribute (Almost all cells migrate bad in serum free conditions). The same non-specific activation will be involved in a chemotaxis assay if u use FCS as chemoattractant. Very often since scratch assays are done with strongly adherent cells, integrin mediated signaling could be predominant. But Integrin based signaling could also strongly affect chemotaxis of certain cells, as seen by strong enhancement of migration through fibronectin / collagen coated membranes.
In addition, independent signaling networks could regulate directional polarisation and force generation for migration, which could lead to strong differences in phenotypes in these two assays.
Hope I didnt confuse u more!!!!
Dear Mathieu,
Your question is really tricky - as it is up to the type of the cell which contributes to the chemotaxis of wound healing. As you know for sure, in regular assays epithelial cells, fibroblasts are used more frequently, however, there are several other references about cells which never show wound healing in physiological conditions.
The most common receptors are chemokine receptors, formyl peptide receptors and extracellular matrix receptors, too. Several growth factor receptors can also trigger the migration in the wound.
I suggest you to visit the homepage of Applied BioPhysics - ECIS is an impedimetry based equipment which can measure wound healing in hightech level (in my lab ECIS wound healing is running). So, I suggest you to visit the ABP homepage and check the wound healing references for receptors.
If you have any question please do not hesitate to ask!
Good luck!
Laszlo
I think the first answer is very clear. Same receptor many receptor up to interested in this receptor such as VEGFR-2 or other. but chemotaxis is focus specific cytokine or proangiogenic factor that relate your studies. Please search in Pubmed If I don't forget it Springer link journal search with Angiogenesis in woundhealing and Angiogenesis assay . I will find it for you. Wait me 1 or 2 days. Chemotaxis is a chemical that specific have effect to this process.But wound healing is many proangiogenesis so needed to reduce grotwh factor.In case of my project interested in VEGFR-2 so I used VEGF as a inducer.
- Badges
- Science topic
- Similar topics
- Biological Science
- Cell Biology