Which IHC marker can we use to differentiate gastric cancer from normal gastric tissue ?

I have some parraffin blocks with cells extracted from gastric cancer patient and healthy patients. So far, I did not find a marker which was exclusively expressed in gastric cancer. Does anybody...

07 August 2016 7,068 3 View

How to remove lipids from primary tissue cell suspension ?

I am trying to extract cancer cells from human gastric cancer tissue. I noticed that, after digesting the tissue, I have a lot of lipids present in my cell suspension. When I try to pellet down...

06 July 2016 4,774 3 View

Could you help me identify these cells isolated from a lung cancer xenograph ?

These cells were isolated from lung cancer xenograph. I have been culturing them in DMEM/F12/B27/FGF for over a month now. They looked like fibroblasts to me but they are forming colonies. Thank...

02 March 2015 1,644 6 View

Is there any scenarios where cells that have a higher ability to invade can posses a higher adhesion potential?

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03 April 2014 688 5 View

How miRNAs get secreted by cells ?

Can we block specifically their secretion ?

04 May 2013 7,267 8 View

Stable tumor suppresor miR clones

Is it possible to obtain a tumor suppressor resistant population from stable transfection with a tumor suppressor miRNA? If the miRNA is supposed to slow proliferation and induce apoptosis, then...

31 December 2012 1,647 4 View

Which cell receptors are involved in a wound healing assay? How different are they from the cell receptors used during chemotaxis?

I understand that wound healing requires cell migration and cell proliferation. I would like to know which cell receptors are activated during the cell migration part of the wound healing assay?...

06 July 2012 1,514 3 View

Do cancer cells switch off their proliferation ability during invasion?

I am wondering if cancer cells invading their micro-environment have the potential to proliferate as they invade or cancer cells can only either invade or proliferate. Thanks

04 May 2012 6,789 18 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View