Mathieu Derouet

9 Questions 12 Answers 0 Followers

Questions related from Mathieu Derouet

What are these vacuoles formed by colorectal cancer organoids ?

After 1 month of culture, I observed the formation of vacuoles adjacent of the organoid (see pictures). Does anybody saw them in their organoid culture or knows what they are ? Thank you

01 January 2017 2,255 2 View

Which IHC marker can we use to differentiate gastric cancer from normal gastric tissue ?

I have some parraffin blocks with cells extracted from gastric cancer patient and healthy patients. So far, I did not find a marker which was exclusively expressed in gastric cancer. Does anybody...

08 August 2016 7,142 3 View

How to remove lipids from primary tissue cell suspension ?

I am trying to extract cancer cells from human gastric cancer tissue. I noticed that, after digesting the tissue, I have a lot of lipids present in my cell suspension. When I try to pellet down...

07 July 2016 4,821 3 View

Could you help me identify these cells isolated from a lung cancer xenograph ?

These cells were isolated from lung cancer xenograph. I have been culturing them in DMEM/F12/B27/FGF for over a month now. They looked like fibroblasts to me but they are forming colonies. Thank...

03 March 2015 1,697 6 View

Is there any scenarios where cells that have a higher ability to invade can posses a higher adhesion potential?

04 April 2014 747 5 View

How miRNAs get secreted by cells ?

Can we block specifically their secretion ?

05 May 2013 7,325 8 View

Stable tumor suppresor miR clones

Is it possible to obtain a tumor suppressor resistant population from stable transfection with a tumor suppressor miRNA? If the miRNA is supposed to slow proliferation and induce apoptosis, then...

01 January 2013 1,695 4 View

Which cell receptors are involved in a wound healing assay? How different are they from the cell receptors used during chemotaxis?

I understand that wound healing requires cell migration and cell proliferation. I would like to know which cell receptors are activated during the cell migration part of the wound healing assay?...

07 July 2012 1,575 3 View

Do cancer cells switch off their proliferation ability during invasion?

I am wondering if cancer cells invading their micro-environment have the potential to proliferate as they invade or cancer cells can only either invade or proliferate. Thanks

05 May 2012 6,840 18 View