I use this chambered culture well plates for immunofluorescent confocal microscopy: https://us.vwr.com/store/product/12361327/culturewelltm-removable-chambered-coverglass-electron-microscopy-sciences
I'm having issues removing the rubber gasket without breaking the attached slide. The slide is very fragile no matter what method I use to remove the gasket. I end up cracking the whole slide and rendering my sample useless.
Does anyone have any suggestions of a technique or a better brand of slides? I need ones that are for small infection volumes (30 ul).
It should come with a removal tool, which I and my colleagues nickname the "cheese slicer". It's just a prong with a filament running between the prongs. You slip it in between the gasket and the slide and coverslip and then pull it through. Did you try this? Other than this, I don't know a way to remove it.
You can also contact Grace Biolabs for recommendation. Though Thermo Fisher Scientific sells them, they are actually made by Grace. www.gracebio.com.
I have had some successs treating it like a protein gel asset. I use a small spatula get in between the seal and then slowly twist to crack the well from the slide. You may need to do that in a few spots before fully detaching the wells. This has worked most of the time, but I have had a few break on me if I use too much force. I hope that helps.
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