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Questions related from Hannah Lynne Murphy
Does anyone know what microbe we are dealing with in our cell culture lines? We are thinking yeast but are not sure. Vero cells seem very susceptible to it.
05 May 2023 8,069 4 View
I am inserting an MPP5 gene insert into a pCAGGs backbone. When I am finished with the mini prep, I find that my RE digestion is correct, but then when I send it to be sequenced, the MPP5 gene is...
24 November 2020 8,103 2 View
I have infected BHK21 cells with a virus that expresses GFP. I want to count the percentage of GFP positive cells and the intensity of the GFP. How do I do this with fluorescent microscope...
18 August 2020 4,547 2 View
What reagent do you use to dilute your cell lysates after finding protein concentration with Bradford assay? Do you use 1X loading buffer or do you use water? If you use the 1X loading buffer to...
17 January 2020 2,593 3 View
I am not getting any bands after miniprep and restriction digestion. I have measured the DNA concentration with the nano drop and my concentrations are all over 80 ng/µL. I restriction digested my...
20 December 2019 7,650 1 View
