How to reduce thiol DNA with DTT?

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Salar Khaledian

in my opinion TCEP-HCl is better than of DTT for reduction process.

Saranya .v

thank you so much Mr.salar khaledian.

Misha Soskine

we used 10 mM DTT in TAE for 1 hr.

I'm not sure what aggregating - gold particles or DNA.

Assuming that you meant gold - make sure that your

DNA is not self-complementary and you don't bridge

the particles together.

thank you Misha, actually how to extract DTT from DNA.

Jie Zhang

Use the NAP-5 Columns from GE healthcare

here is a handbook

thank you jie.

Muhammad Nur Hasan

You can read this protocol for sure..

https://www.nature.com/articles/nprot.2006.38