What pH condition required to coat APTES on silica coated Fe3O4?

It's a hydrolysis reaction, which may produce by either acid or base-catalyzed reaction. so can anybody help me with this, which condition is suitable for this reaction?

26 February 2021 1,760 8 View

What condition required for coating of Fe3O4@SiO2 by using APTES?

I tried coating Fe3O4@ SiO2 by using APTES in 5% water-ethanol but it is not coated. Kindly suggest a solution for it. Thanks

24 February 2021 5,992 4 View

How to Control Whiteflies in coconut leaves?

24 February 2021 1,005 1 View

Is it possible to design digital all pass filter for specific frequency range of the system?

I would like to know, Is it possible to design digital all pass filter for specific frequency range of the system to alter the phase of the system in that specific range of frequency? If yes how...

26 November 2020 1,402 5 View

In some CP patch design the polarization changes for LHCP to RHCP, What should be the possible reasons?

I have designed a dual print dual cp antenna, identical patch on both side of the substrate (placed side by side), on analysis, it is found it is showing LHCP in one band and RHCP in another band....

04 August 2020 5,136 3 View

In dual band antenna why the gain in upper band decreases?

In some printed antenna designs, generally, the gain in the upper band is larger than the lower band. However, in some cases (in my design having printed circle on both side of the substrate like...

03 August 2020 5,802 5 View

How to cap the maximum amount of reinforcement addition to make a polymer to be thermally conductive?

Addition of reinforcement with very high thermal conductivity (ex. graphene) would enhance the thermal conductive value of the developed polymer composites. How would one cap the maximum weight...

21 June 2020 9,303 11 View

We are facing rotten problem in kesar mango near mouth of it. What may be reason for that?

We are doing natural farming of kesar mango. This year we are facing rotten problem in mango near mouth of it. Last year it seems good. For this reason we are facing problem in selling if it....

02 June 2020 5,277 7 View

In COVID-19 modeling estimates for India by CDDEP, Johns Hopkins, and Princeton, what decideds peaking and decay?

COVID-19 modeling estimates for India have been presented by a team of researchers affiliated with CDDEP, Johns Hopkins, and Princeton. The results described in...

28 March 2020 1,623 2 View

Up to what temperatures can nCovid-19, novel corona virus survive?

The question does not require much explanation. Interested to know if there is some study on overall pattern of temperature range of permanent inactivity of various viruses and bacteria of all...

10 March 2020 8,749 44 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

How to compare different concentrations of nanoparticles for characterization from ICP-MS?

I took measurements of different concentrations of 15 nm and 5 nm Au nano particle solutions. I'm trying to determine which concentration I measured gives the best reading overall. I also...

02 March 2021 7,626 1 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

Best plasmid could be used as a standard curve for AAV titration?

I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...

23 February 2021 5,167 3 View

How does the injection volume impact LLOQ and ULOQ in regression analysis (LC-MSMS)?

Hello, I have run into an issue that has sparkled a debate at work: is the LLOQ/ULOQ impacted by injection volume under the following circumstances: 1) Same dilution factor of all samples 2)...

23 February 2021 9,159 3 View