We are extracting outer membrane proteins from gram-negative bacteria as vaccine candidate antigens. However, we did not know how to quality control of bacterial outer membrane proteins. As we all know, outer membrane protein complex (OMPC) of Neisseria meningitidis group B had been used as carrier protein of carbohydrate conjugate vaccine, such as PedvaxHIB. In addition, there are other bacterial outer membrane proteins that have entered clinical trials as vaccine antigens. In those cases, how to quality control of bacterial outer membrane proteins? Thank you very much.
The OMPs (outer membrane proteins) of Gram-negative bacteria have to be translocated through the periplasmic space before reaching their final destination. The aqueous environment of the periplasmic space and high permeability of the outer membrane engender such a translocation process inevitably challenging. In Escherichia coli, although SurA, Skp and DegP have been identified to function in translocating OMPs across the periplasm, their precise roles and their relationship remain to be elucidated.
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