How to purify genomic DNA following DpnI treatment?

More Pouria Akhbari's questions See All

What is the best method for miRNA expression analysis in Rat hippocampus?

Hello to all. I am performing a thesis on Cognitive disorder modeling in rats and the effect of Pharmacological Treatment on the disease and the expression of certain miRNAs. I am seeking...

14 October 2020 6,690 1 View

Is there any need to Do PCR After RT-PCR?

Hello. I would be thankful if anyone could answer my question. We are Analyzing the expression of a certain gene in our research. We have extracted all the mRNAs in the cell and now I was...

22 July 2020 768 4 View

No title

As you may know, blocking the receptor binding domain (RBD) of novel coronavirus could be very effective to prevent more severe effects of the inflammation. So, can a BsAb co-targeting CD8+ t...

09 April 2020 8,900 1 View

What is a proper way to clean the TiO2 mesoporous electrode?

the immobilization enzyme on TiO2 mesoporous layer.

12 October 2019 1,856 2 View

What are the best targets (genes) for radio sensitization in crc?

I'm about to knockdown some target genes to increase radio sensitization in colon cancer cell lines, so what are the most potential genes?

24 January 2017 1,085 2 View

How can I find Building stone like travertine and marble?

Hello dear friends. I want to know how can I find Building stones like Travertin and Marble according Remote Sensing and geochemistry anomaly or ....Actually in Iran we find Building Stones by...

06 August 2016 8,735 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Do you know good and user-friendly programs to measure plant quantitative traits?

For my research i will need to measure plant quantitative traits (especially leaves area and roots length, but would be nice to add some more information). I recently discovered...

01 March 2021 5,035 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Tersagh Smart Ichor

use DpnI immobilized on magnetic beads. The ten minute extraction technique allows specific binding of genomes containing the DpnI Gm6ATC motif common in the genomic DNA of many bacteria including γ-proteobacteria. Using synthetic genome mixtures, you can recover genomic DNA even when only femtogram quantities are spiked.

Pouria Akhbari

Thanks very much Tersagh. Will try it.

Lưu NGỌC Sinh

thanks