What is the best method for miRNA expression analysis in Rat hippocampus?

Hello to all. I am performing a thesis on Cognitive disorder modeling in rats and the effect of Pharmacological Treatment on the disease and the expression of certain miRNAs. I am seeking...

14 October 2020 6,690 1 View

Is there any need to Do PCR After RT-PCR?

Hello. I would be thankful if anyone could answer my question. We are Analyzing the expression of a certain gene in our research. We have extracted all the mRNAs in the cell and now I was...

22 July 2020 768 4 View

No title

As you may know, blocking the receptor binding domain (RBD) of novel coronavirus could be very effective to prevent more severe effects of the inflammation. So, can a BsAb co-targeting CD8+ t...

09 April 2020 8,900 1 View

What are the best targets (genes) for radio sensitization in crc?

I'm about to knockdown some target genes to increase radio sensitization in colon cancer cell lines, so what are the most potential genes?

24 January 2017 1,085 2 View

How to purify genomic DNA following DpnI treatment?

02 October 2016 9,040 3 View

How can I find Building stone like travertine and marble?

Hello dear friends. I want to know how can I find Building stones like Travertin and Marble according Remote Sensing and geochemistry anomaly or ....Actually in Iran we find Building Stones by...

06 August 2016 8,735 3 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

How to define and get results (stresses) for certain points of a model in abaqus, using python? When the size of the model changes?

I want to do a parameter study of a aixsymmetric cylinder in Abaqus. I want to create several jobs where I get the stresses for 3 different points in my model. These points are defined. I wrote a...

01 March 2021 10,078 1 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

Joint behaviour in Abaqus is not captured well?

I am working on roof joint and trying to simulate the behaviour in Abaqus. I am successful in running the model but the final graph does not follow the experimental curve in the negative direction...

01 March 2021 6,661 3 View

Which one is easier and better at modeling slender masonry structures? ANSYS or ABAQUS?

I need to model masonry and reinforced concrete minarets for my MSc thesis. Which one is easier and better at modeling slender masonry structures? ANSYS or ABAQUS? (Or any other program for just...

01 March 2021 736 3 View

How to assign different material properties to each element in Abaqus ?

Hello, I am writing a python script to generate my model and write the input file. The problem is I have 1000 elements in my mesh and I want to assign different material properties to each...

01 March 2021 9,511 3 View

How to define tie constraint between rigid body and another part in dynamic explicit model of Abaqus?

Hi dear researchers I connected a rigid body into the part of my structure by tie constraint and then I added displacement to rigid body, in static general step every thing is fine and these two...

28 February 2021 3,442 2 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

How to modify Johnson cook equation in ABAQUS?

I wanted to add an extra parameter to the existing Johnson cook equation in the ABAQUS simulation. How to add those extra parameters. Ref: A modified Johnson-Cook material model with strain...

28 February 2021 4,841 3 View

For modelling epoxy-based matrices (in composites), which model in ABAQUS is the most suitable, Concrete damaged plasticity or Drucker-Prager model?

I am working on developing a micromechanical FE model for predicting kink-band formation in UD composites. To model matrix plasticity, which model, out of Concrete Damaged Plasticity and...

28 February 2021 5,415 1 View