Hi. Im working on HepaRG gells and seeding them on MatTek glass bottom dishes for TUNEL assay. The MatTek dishes are uncoated. The proliferative cells is adhering to the plate pretty well. But when I'm differentiating the cells in MatTek dishes, my cells are coming off from the glass part of the dish. But in the plastic part, it looks ok. What adherent aid can be used for differentiated HepaRG cells so that it can stick to the glass well?
To increase cell adherence to glass surfaces, differentiated HepaRG cells can be coated with different substances. There are several coating choices, including collagen, fibronectin, poly-L-lysine, gelatin, and laminin. Fibronectin improves cell attachment, whereas collagen is a protein that encourages cell adhesion. A synthetic covering for adherent cell cultures is called poly-L-lysine. Collagen is the source of gelatin, which is employed in tissue culture to enhance cell adhesion. Cell adhesion is further enhanced by the glycoprotein laminin found in the extracellular matrix. For HepaRG cells on MatTek glass bottom dishes, the best coating can be identified through a small-scale optimization experiment.
https://onlinelibrary.wiley.com/doi/abs/10.1002/eji.1830190521
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0112122
https://benthamopen.com/ABSTRACT/TOBIOTJ-2-133
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