I am not able to find proper procedures for staining and how to prepare the slides for SEM. Kindly suggest protocols or any articles.
(I want to study Aspergillus morphologically).
Please try this:
https://s3-us-west-2.amazonaws.com/awprotocols/17656764.pdf
page 456, they describe the procedure for biological specimen, aspergillus included. Hope this helps.
Additional sources:
Article A Preparation Method of Aspergillus spp. for Scanning Electr...
https://link.springer.com/chapter/10.1007%2F978-1-4614-2356-0_8
DOI https://doi.org/10.1007/978-1-4614-2356-0_8
Respected sir,
Thank you for your kind help. Przemyslaw Wachulak
Regards
Mr. Anup Kodape
Dear,
You can make fixation by Glu. and Parfor. vapor inside the petri dish and after 1 h you can put it on stub , best examination by FESEM low voltage better than SEM
Thank you, Sir Rafid Doulab for providing new information.
Could you please elaborate what do you mean by this:
"how to prepare the slides for SEM"........
Pl check the link given by Wachulak.
I will give you some more ideas if you explain the following:
Is your sample grown in agar media, in suspension, or in any other form. It's not clear in your question.
or we can collaborate
01 August 2024 6,705 2 View
Historical building polygon shapefile
27 September 2023 8,273 1 View
What are the geometric and physical interpretations of fractional-order derivatives in mathematical models?
25 May 2023 7,893 1 View
25 May 2023 4,067 2 View
Hi, I am trying to design a lag damper for articulated helicopter rotor. Could someone provide some technical details/documents/papers a preliminary design of hydraulic/hydro-elastic...
10 December 2022 8,013 0 View
I want to prepare an Essential oils standard solution of (2.5, 5, 7.5, 10, 25, 50 & 100 ppm) from the Potato Dextrose Broth stock solution (1000ppm). Kindly suggest how shall i prepare it or...
06 October 2022 8,229 3 View
Generally in BOD test for sewage water, it is prescribed to use distilled water for dilution along with addition of various nutrients viz. phosphate buffer, magnesium sulfate solution, calcium...
27 May 2022 3,942 3 View
I am comparing the effect treatment vs no treatment on health outcome in my retrospective longitudinal study. As subjects were probably not randomly allocated to the treatment group, I am using...
21 March 2022 9,552 2 View
Hi, I would like to know any other device we can use for calibration of FBG sensor apart from micro UTM
09 August 2021 8,469 3 View
i would like to know the methodology
02 August 2021 2,560 0 View
I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?
11 August 2024 3,115 1 View
I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.
11 August 2024 9,101 4 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...
10 August 2024 7,180 0 View
I am developing a predictive model for a water supply network that involves 20 influencing points. However, I only have historical data for 10 out of these 20 points. I would like to know how to...
10 August 2024 4,005 2 View
I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...
10 August 2024 7,429 2 View
Usually, additive manufacturing techniques like SEBM, SLS, and SLM are used for interconnected porous lattice structure generation with sizes of >100–200 micrometers. Can the Fused Deposition...
09 August 2024 7,892 0 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
I need to model an anisotropic material in which the Poisson's ratio ν_12 ≠ ν_21 and so on. Therefore, the elastic compliance matrix wouldn't be a symmetric one. In ANSYS APDL, for TB,ANEL...
09 August 2024 5,048 2 View