I am not able to find proper procedures for staining and how to prepare the slides for SEM. Kindly suggest protocols or any articles.
(I want to study Aspergillus morphologically).
Please try this:
https://s3-us-west-2.amazonaws.com/awprotocols/17656764.pdf
page 456, they describe the procedure for biological specimen, aspergillus included. Hope this helps.
Additional sources:
Article A Preparation Method of Aspergillus spp. for Scanning Electr...
https://link.springer.com/chapter/10.1007%2F978-1-4614-2356-0_8
DOI https://doi.org/10.1007/978-1-4614-2356-0_8
Respected sir,
Thank you for your kind help. Przemyslaw Wachulak
Regards
Mr. Anup Kodape
Dear,
You can make fixation by Glu. and Parfor. vapor inside the petri dish and after 1 h you can put it on stub , best examination by FESEM low voltage better than SEM
Thank you, Sir Rafid Doulab for providing new information.
Could you please elaborate what do you mean by this:
"how to prepare the slides for SEM"........
Pl check the link given by Wachulak.
I will give you some more ideas if you explain the following:
Is your sample grown in agar media, in suspension, or in any other form. It's not clear in your question.
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