Fraction collector Frac-920 not working with AKTA?

Hi, I routinely use AKTA connected to Fraction Collector-920 for collecting proteins while doing protein purification. However, recently I have started seeing some errors on our Fraction...

27 February 2020 9,025 4 View

No colonies from NEB high-efficiency chemically competent cells?

Hi all, I am doing a few site-directed mutagenesis reactions and after digestion of the parent plasmid using Dpn1, I add the complete 50 uL reaction volume to 50 uL of NEB chemically competent...

01 November 2019 3,085 3 View

Low plasmid miniprep yields?

Hi, I have been working on plasmid minipreps using Qiagen miniprep kit and I have run into weird problems of low plasmid yields. When we purchased a new kit, then my plasmid concentrations were...

12 March 2019 9,028 3 View

What could be the probable mechanism underlying the reduction in body weight of L-NAME treated rats?

What could be the probable mechanism underlying the reduction in body weight of L-NAME treated rats when compared to Control rats?

14 September 2018 6,964 2 View

Can anybody help to extract features of apple???

How the statistical features can be extracted

07 April 2018 2,720 3 View

How to avoid sticking of solution to the walls of 96-well plate ?

As a part of my process, I need to freeze my protein solution in 96-well plate and then freeze dry it. I need to remove the solution intact to study it further. But the scaffolds stick to the wall...

05 December 2017 4,798 1 View

Does anyone have expertise using Getis-Ord hotspot analysis?

14 March 2017 541 2 View

Calculating nanomoles with knowing only conc and OD?

Hi All, I am wondering if we can calculate the nanomoles of RNAi, knowing only details of its ug/ul concentration and OD values; though no information of basepair is available? please suggest! Best,

02 February 2017 432 3 View

Is there any empirical relation to deduce mineral N in the soil layer on the basis of Available nitrogen?

Mineral N (nitrate and ammonical form) is highly dynamic, and is generally not measured by most of the researchers, who most of the time take total N or available N in consideration for evaluating...

28 January 2016 6,998 5 View

Several soil quality indices reported in literature, but effective operational soil health service missing. Please suggest the ways.

1. Lot of information on soil test values (spatio-temporal)) available 2. Soil quality indices e.g. SQI by Ratan Lal et al, Cornell soil health index, STCR network ar Indian Institute of Soil...

21 January 2016 8,570 26 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Samples to study circular dichroism (chirality)?

I want to build a low-cost polarimeter to study circular dichroism (chirality). What type of samples can I use? I am looking for samples that can be found outside the laboratory.

01 March 2021 9,101 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

can I treat waste water containing solvents and alcohol with electrocoagulation?

there are solvents and alcohol in the waste water of a factory that produces disinfectants. Increasing this waste water requires very serious operations. Can I treat this waste water with...

27 February 2021 2,609 3 View

Can anyone help with Schistosoma identification?

Can anyone identify what kind of Schistosome species this might be? Morphological features of cercariae and adults resemble Trichobilharzia, though I am fairly new to the field of parasitology...

25 February 2021 7,760 6 View

How vulnerable is water and wastewater infrastructure to cyber terrorism in an age of IoT?

Im looking for any papers, reports,articles, links etc on information on How vulnerable is water and wastewater infrastructure to cyber terrorism in an age of IoT.

24 February 2021 2,750 4 View

Suppose we have a banana seller cart consisting of 4 wheels, weighing about 300 kg. How much torque motors are needed to pull of push it electrically?

I am thinking of electrifying the street food carts while harvesting energy using solar panels on top.

22 February 2021 4,323 3 View

Does anyone have any experience with the Motic EasyScan Pro Slide Scanner?

I am interested in purchasing a slide scanner for histology and was presented with the Motic EasyScan Pro but I have never heard of the company, nor know of anyone who has used the system. Can...

11 February 2021 2,416 2 View

Do the introduced salts (NaOH/ HCl) influence the spectroscopic shifts of the fluorescence excitation-emission matrix?

During the resin (XAD-8) fractionation of wastewater salts (NaOH or HaCl) are used for acid or base elutions/pH adjustments. Since the amount of introduced salt is minimal, so is it required to...

08 February 2021 9,489 2 View