https://www.neb.com/products/m0271-quick-load-taq-2x-master-mix#Product%20Information

Hi Nasim,

you can effectively do your own PCR mix for qPCR testing (IGEPAL is distributed by some professionals as SIGMA (https://www.sigmaaldrich.com/catalog/product/sial/i3021?lang=fr®ion=FR&gclid=Cj0KCQiA8_PfBRC3ARIsAOzJ2up3n2XdwqWihFvCu3Qq8VHVCyD7thymLX2xDDBGo4uJRMxAvk_O9scaAnxMEALw_wcB)). The problem is the reproducibility of your preparation first, and the cost of all separated chemicals.

As Jia-Wang precised, see all elements, their cost and see the price of a kit, I think there is no doubt, the second version will be more reliable and less expensive.

fred

Hey,

and even a formulaiton for a qPCR system may look different as intercalating dyes might have some additional requirements.

Thank you all for your responses. So, the best thing to do is trying ready to use SYBR master mix instead of making it manually I guess?

I would recommend buying a pre-made SYBR mix for consistency.