9 Questions 39 Answers 0 Followers
Questions related from Martin Somoza
Hello, I am planning to do a cytokine immunoflourescence in spleen tissue slices. In bibliography most methods do not permeabilize cells, thereby detecting extracellular protein (associated to...
06 June 2018 9,523 3 View
Hello everyone, Is there known antibody to block BCR function? Or any knock out mouse strain deficient in BCR signalling? Thanks in advance, Martin
02 February 2018 8,849 3 View
Hi, I can't find anywhere what is the T helper profile induced by pOVA(323-339) in DO11.10 splenocytes. I was wondering what is the "starting point" of the cytokine profile using this model to...
07 July 2017 6,985 1 View
Hi, I'm currently working with an enzymatic reaction. The reagents are quite expensive, so I wonder if I can scale down the reaction volume from 100ul to 25ul. Is this possible or there is any...
06 June 2017 3,723 6 View
Hi, I´ve been staining SDS page gels using the silver reduction technique. The main goal is to cut off differential bands (between treatments) from the gel and analyze it by MS. After purification...
03 March 2017 3,987 3 View
Hello, I've been comparing intracellular staining protocols and noticed that there are several alternatives for fixation with paraformaldehyde, varying time and concentration. Also, I read that...
12 December 2016 663 5 View
Hi, I'm having trouble trying to stain mouse IL-4 from Th2 cells polarized in vitro.I've been purifying CD4+ cells from BABL/c spleens with magnetic beads and culturing them in CD3/CD28 pre-coated...
11 November 2016 8,661 8 View
Hi, i tried acetone precipitation but they're not soluble. Anyone knows another method compatible with high concentrations of guanidine? Thanks
10 October 2016 2,690 4 View
Hello, I've been having trouble with biotynilated protein purification using Avidin-Agarose resin. The idea is to use the purified biotynilated molecules as samples for MALDI-TOF-MS.To check the...
07 July 2016 2,940 6 View
