I used to prepare DMEM-powdered media for the cell culture use. The last step of media preparation is media filtration using 0.2um filters. Meanwhile, in case of 0.2um filters shortage, is it possible to alternatively prepare a sterile media for the cell culture without using 0.2um filters. Like to use 0.45um filters and then autoclave the media to ensure the sterile condition of the prepared media?
Hello Jamila Hijazi
You may autoclave culture media prepared from powder (I would not suggest autoclaving), but sterile filtration through 0.2um or 0.22um filter is recommended, particularly if serum or other supplements are to be added before use.
The use of 0.45-micron filter to filter sterilize the culture media is not advisable because bacteria are still capable of passing through a 0.45-micron filter in large quantity. The 0.45-micron filter is typically used for general filtration and particle removal while 0.2/0.22um filter is most used for solution sterilization (bacteria removal).
So, make use of 0.22um filter to filter sterilize your culture media in order to obtain good results.
Best.
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