Jamila Hijazi

5 Questions 7 Answers 0 Followers

Questions related from Jamila Hijazi

How to Prepare Powdered-media for cell culture in case we lack 0.2um filters?

I used to prepare DMEM-powdered media for the cell culture use. The last step of media preparation is media filtration using 0.2um filters. Meanwhile, in case of 0.2um filters shortage, is it...

29 February 2024 6,240 1 View

How can we improve the detection sensitivity of Western Blotting for proteins with low signal levels?

I have conducted several western blots to detect the expression of Zonula Occludens-1 (ZO-1), NCX-1, and PMCA1. However, I have observed that the expression levels of these proteins are very low,...

19 February 2024 3,040 7 View

How can we address the variability in PCR analysis stemming from differing Cq values observed in the reference gene?

I have conducted three replicates of samples using quantitative PCR. Each sample shares the same conditions, with the only variation being the cell passage. Although I obtained similar Cq values...

19 February 2024 1,662 5 View

What would be the ultimate way to test the specificity of primers before performing PCR?

I have selected a set of primer sequences for my RT-PCR experiments, using either the NCBI Primer Blast software or Primer3. To ensure the specificity of these primers before ordering, I employed...

06 June 2023 6,694 3 View

What is the best sample collection protocol for western and RNa experiments?

I'm culturing Caco2 cells on a two 6-well plate inserts, one for Rna experiment and the other one for western analysis. Knowing that I want in the first place to collect my samples and store them...

02 March 2023 9,257 2 View