Analysis of hypericin by HPLC in food matrix?

Hello! Friends I need to analyze naturally occurring toxic substances Hypericin in Food matrix. Can somebody suggest a suitable HPLC or other method for that? Your inputs will highly be...

09 October 2018 358 0 View

Unknown concentration calculation using HPLC?

A typical formula often used for the concentration of an unknown sample concentration as follows: Conc in ppm= (standard area/ sample area)* stnadard conc.* Dilution factor Can anybody derive...

06 July 2018 9,580 6 View

Quantitative determination of Ascorbyl stearate in Margarine or any Oil sample?

Please provide me any suitable methods if any for the quantitative determination of ascorbyl stearate in Margarine or any Oil related sample.

02 March 2018 8,108 1 View

Why octahedral site of spinel ferrite is more active for any catalytic reaction than that of tetrahedral site?

02 March 2016 6,550 1 View

Is it possible to separate peptides and amino acids by using HPLC equipped with a UV-visible detector?

I want to separate glycine and its oilogomer with hplc having uv-vis detector at wavelength of 195 nm but i failed to do that. Can anyone suggest some suitable method to do that?

31 December 2015 2,837 4 View

Is it possible to separate peptides and amino acids by using HPLC equipped with a UV-visible detector?

Is it possible to separate peptides and amino acids by using HPLC equipped with a UV-visible detector?

04 May 2014 8,753 1 View

Can anyone tell me the zero point charge value (pzpc) of molybdenum oxides?

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11 December 2013 8,961 1 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

Which database would give me this list of chemical components?

Dear colleagues: I would like to know if anyone knows of a database that gives me the parameters pH, K, K, Mg, P, C, N, Zn, Cu, Ni and Cd of common foods. I find a lot of information of proteins,...

26 February 2021 5,525 3 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Why is NH4OH added in heavy metals limit test?

The limit test I'm referring to is USP method II. In this procedure you need to dropwise add 6N of NH4OH to the sample until it the pH is alkali. After that you need to dilute and add HOAc until...

23 February 2021 2,049 3 View