I want to prepare 0.05 M phosphate buffer of pH 7 from Na2HPO4 and KH2PO4. How to proceed?
Hello! Friends I need to analyze naturally occurring toxic substances Hypericin in Food matrix. Can somebody suggest a suitable HPLC or other method for that? Your inputs will highly be...
09 October 2018 406 0 View
A typical formula often used for the concentration of an unknown sample concentration as follows: Conc in ppm= (standard area/ sample area)* stnadard conc.* Dilution factor Can anybody derive...
06 July 2018 9,711 6 View
Please provide me any suitable methods if any for the quantitative determination of ascorbyl stearate in Margarine or any Oil related sample.
02 March 2018 8,149 1 View
The catalytic properties of ferrospinels crucially depend on the distribution ofcations among the octahedral and tetrahedral sites of the spinel structure and the catalytic activity was mainly due...
02 March 2016 6,599 1 View
I want to separate glycine and its oilogomer with hplc having uv-vis detector at wavelength of 195 nm but i failed to do that. Can anyone suggest some suitable method to do that?
31 December 2015 2,882 4 View
Is it possible to separate peptides and amino acids by using HPLC equipped with a UV-visible detector?
04 May 2014 8,782 1 View
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11 December 2013 9,026 1 View
Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?
07 August 2024 4,616 0 View
Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...
06 August 2024 5,373 2 View
Hello, I suspect that a molecule I am using is causing bacteria to experience osmotic shock. What chemical properties should I look for to compare this capability with those of other substances?...
06 August 2024 977 1 View
Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...
05 August 2024 3,783 2 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View
We are working on biopolymeric hydrogels. Our system is highly viscous and sticky, and the gel formed are high in strength. We are unable to use pH electrode and pH strip. Please suggest an easy...
30 July 2024 942 2 View
Hello everyone! How can i prepare EDTA buffer solution (pH 8.2) to determine GSH levels? Thank you in advance.
29 July 2024 4,374 1 View
I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.
29 July 2024 8,560 0 View
// interested in the difference between floating events and short circuits.
22 July 2024 6,565 0 View
Our lab has extracted bacterial DNA samples using Qiagen DNeasy kit and eluted the samples in AE buffer. However, this buffer contains 0.5mM EDTA not suitable for downstream application. Any ideas...
13 July 2024 1,675 3 View