How to prepare phosphate buffer?

I want to prepare 0.05 M phosphate buffer of pH 7 from Na2HPO4 and KH2PO4. How to proceed?

10 November 2018 6,555 0 View

Unknown concentration calculation using HPLC?

A typical formula often used for the concentration of an unknown sample concentration as follows: Conc in ppm= (standard area/ sample area)* stnadard conc.* Dilution factor Can anybody derive...

06 July 2018 9,580 6 View

Quantitative determination of Ascorbyl stearate in Margarine or any Oil sample?

Please provide me any suitable methods if any for the quantitative determination of ascorbyl stearate in Margarine or any Oil related sample.

02 March 2018 8,108 1 View

Why octahedral site of spinel ferrite is more active for any catalytic reaction than that of tetrahedral site?

02 March 2016 6,550 1 View

Is it possible to separate peptides and amino acids by using HPLC equipped with a UV-visible detector?

I want to separate glycine and its oilogomer with hplc having uv-vis detector at wavelength of 195 nm but i failed to do that. Can anyone suggest some suitable method to do that?

31 December 2015 2,837 4 View

Is it possible to separate peptides and amino acids by using HPLC equipped with a UV-visible detector?

Is it possible to separate peptides and amino acids by using HPLC equipped with a UV-visible detector?

04 May 2014 8,753 1 View

Can anyone tell me the zero point charge value (pzpc) of molybdenum oxides?

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11 December 2013 8,961 1 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

I have designed reflectarray element and feeding it though Horn Antenna on HFSS. After full wave simulation, I am not getting desired results?

I have designed reflectarray element to generate OAM beams at 5.8GHz and feeding it though Horn Antenna on HFSS. After full wave simulation, i am not getting desired results i.e Radiation pattern...

01 March 2021 9,701 5 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Microstrip antenna design tutorial of HFSS 3D layout module ?

I have designed a 24 GHz rectangular antenna on HFSS 3D model and got desired results while desing the same antenna with same dimensions I am getting resonance frequency at 27 GHz I am...

28 February 2021 4,426 2 View