Hello everyone,
I am doing some antioxidant assays and for that I need to prepare acetic acid buffer with pH 4.5, I searched a lot about this but can’t found an answer which I can understand easily because every answer is different from each other. I would like to request you all please help me that how can I prepare acetic acid buffer with pH 4.5.
Thank you
It would be a good idea to find a calibrated pH meter to measure the pH of the solution.
You can also use it to prepare the solution. A method I advocate if a pH meter is available goes like this: First, decide on what concentration of acetate you want the buffer solution to have. Then prepare 2 solutions: acetic acid and sodium acetate at that concentration. Get a magnetic stirrer and stir bar and set up one of the solutions on the stirrer in a beaker with plenty of headroom and suspend the calibrated pH electrode in the liquid. Start measuring the pH and add the second solution a little at a time until the pH is 4.5.
pH 4.5 (0.2 M Sodium Acetate Solution): Weigh 27.20 g of Sodium Acetate Trihydrate into a one liter volumetric flask. Add 800 mL of deionized water. Mix and dissolve. Bring the pH down to 4.5 with Glacial Acetic Acid. Finally adjust the volume to one liter with deionized water to obtain 1000 mL of solution having a pH of 4.50 ± 0.05.
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