I would like to prepare 1 mM solution of polydI-dC (Poly(deoxyinosinic-deoxycytidylic) acid sodium salt). Please suggest me how to do this, here is the product link
http://www.sigmaaldrich.com/catalog/product/sigma/p4929?lang=en®ion=IN
do you get the answer?
With enzymes for example the "specific activity " will be given in units/mg on the vial. The vial is normally supplied with a predetermined volume of enzyme, for example 1ml. You then have to convert mg/ml into units/ml if required. This is a way of quantifying an exact amount of a product into an experiment, as the number of units between enzyme batches vary.
On your vial of TNF alpha there will be a total number of units/vial. Also it should indicate the number of units/mg of TNF. If you know the volume then it is a simple calculation to convert into units/ml.
i.e. Vial of TNF (1mg) = 50,000 units/mg
Volume of liquid = 1ml
Therefore your stock solution = 1mg/ml or 1ug/ul or
50,000u/ml or 50u/ul
Does this help or is it too confusing still.
Kindest regards
Uncle Rhombus
-rhombus-
I had the same problem but, according to https://www.sigmaaldrich.com/catalog/product/roche/polydicro?lang=es®ion=ES , 1 A260 unit corresponds to 50 μg ds DNA.
I hope it will be usefull in the future.
Chus
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