How can I visualize weekly fluorescent dye encapsulation with DOPC lipid?

08 September 2016 2,412 2 View

Hi I would like to aggregate BSA protein in Acidic medium with salt. Any body can help me?

Hi I would like to aggregate BSA protein in Acidic medium with salt. Any body can help me?

31 December 2015 6,577 2 View

What is the Pka of (Hydroxypropyl)methyl cellulose) and how do I prepare the 1:1 stoichiometry of 1 mM soln of HPMC with NaOH soln?

I would like to prepare 1mM stock solution of HPMC with NaOH. The ratio of -CH-OH in HPMC and Na+ should be in 1:1 ratio. Please help me to prepare this solution.

08 September 2014 1,548 0 View

How to prepare 1 mM solution of poly dI-dC from 10 U pack?

I would like to prepare 1 mM solution of polydI-dC (Poly(deoxyinosinic-deoxycytidylic) acid sodium salt). Please suggest me how to do this, here is the product...

08 September 2014 1,472 2 View

Can anybody suggest which type of HPLC column will be useful for separating Di positive carbocyanine dyes?

06 July 2014 9,412 2 View

Can anybody help me to prepare 3x 10 -4 concentrated solution of carboxymethyl cellulose polymer avg Mw 250,000?

Can anybody help me to prepare 3x 10 -4 concentrated solution of carboxymethyl cellulose polymer( avg Mw 250,000)

06 July 2014 1,435 5 View

How can I prepare 0.3 mM of Sodium carboxymethyl cellulose, average Mw ~250,000?

Will it follow the same molarity calculation as we do for small molecules, or is their any different calculations?

06 July 2014 7,222 1 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Can we recognize signs of the "volatility clustering" property of a time series in its time domain plot?

My purpose is to recognize the volatility clustering property without statistical tests such as The Lagrange Multiplier (LM) test.

24 February 2021 4,150 3 View

How to run repeated measures in mvabund?

I have a dataset of fish species abundances collected from 6 different habitats over the course of 30 years. Fish were collected multiple times per year and environmental variables were recorded...

21 February 2021 3,838 1 View

Is there a method for evaluating nanoparticle adhesion to epithelial tissue in vitro?

I want to measure how well nanoparticles can transfect cells based on NP adhesion properties. In the literature I find people do it in animal studies, and epithelial adhesion helps in transfection...

21 February 2021 7,791 3 View

How to determine number concentration of polymer solutions using viscosity experiment?

I have synthesized PNIPAM (Poly (N-isopropylacrylamide)) microgels, which are copolymerized with oligo-ethylene glycol based comonomers. I have to determine the exact number concentration for each...

19 February 2021 5,205 5 View

Any Homemade Organic Synthesis Practices?

Hi everyone, I am a teacher of Heterocycles Synthesis, Is there a home practice that you recommend for undergraduate students? A simulator would also be useful Due to the pandemic, students...

18 February 2021 2,769 4 View