I would like to prepare 1mM stock solution of HPMC with NaOH. The ratio of -CH-OH in HPMC and Na+ should be in 1:1 ratio. Please help me to prepare this solution.
Dear members, Can you please help me out, how to visualize dye encapsulated liposomes. As my dye is weekly fluorescent nature in lipid vesicle will it be useful to take fluorescence or con focal...
08 September 2016 2,510 2 View
Hi I would like to aggregate BSA protein in Acidic medium with salt. Any body can help me?
31 December 2015 6,701 2 View
I would like to prepare 1 mM solution of polydI-dC (Poly(deoxyinosinic-deoxycytidylic) acid sodium salt). Please suggest me how to do this, here is the product...
08 September 2014 1,613 2 View
I purchased poly dA-dT.poly dA-dT 10 unit packs from sigma and the concentration was found spectrophotometrically. I got a weight of this product in ug/ ml but I would like to prepare a uM...
08 September 2014 2,486 6 View
I would like to buy a column for separating my cyanine dyes. Is there any special columns will required to separate the positive charged dyes or C 18 columns will work for positive charged...
06 July 2014 9,504 2 View
Can anybody help me to prepare 3x 10 -4 concentrated solution of carboxymethyl cellulose polymer( avg Mw 250,000)
06 July 2014 1,531 5 View
Will it follow the same molarity calculation as we do for small molecules, or is their any different calculations?
06 July 2014 7,322 1 View
A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...
11 August 2024 9,801 2 View
I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...
08 August 2024 8,329 0 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
How can I measure the thickness of thin film by Uv-Vis?
06 August 2024 7,810 3 View
Why is TLC not a form of partition chromatography and paper chromatography not a form of absorption chromatography?
03 August 2024 7,035 3 View
I need something like Camag's VisionCat software for photodocumentation of TLCs. Could you recommend something?
31 July 2024 8,415 1 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
Hello, I'm trying to measure the conductivity of semiconductor films but since I don't have a commercial four point probe set up I would like to build one on my own in my lab. I have generators,...
30 July 2024 906 2 View
Hi, researchers, Is it possible to use a safer acid instead of monochloroacetic acid to convert bacterial cellulose to carboxymethyl cellulose?
29 July 2024 4,570 0 View
In my molecule there is Chloro group at 2-position of phenyl ring, but in 2D image it appears as methyl showing no interaction.
28 July 2024 734 0 View