Can anybody suggest which type of HPLC column will be useful for separating Di positive carbocyanine dyes?

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How can I visualize weekly fluorescent dye encapsulation with DOPC lipid?

Dear members, Can you please help me out, how to visualize dye encapsulated liposomes. As my dye is weekly fluorescent nature in lipid vesicle will it be useful to take fluorescence or con focal...

08 September 2016 2,510 2 View

Hi I would like to aggregate BSA protein in Acidic medium with salt. Any body can help me?

31 December 2015 6,701 2 View

How to prepare 1 mM solution of poly dI-dC from 10 U pack?

I would like to prepare 1 mM solution of polydI-dC (Poly(deoxyinosinic-deoxycytidylic) acid sodium salt). Please suggest me how to do this, here is the product...

08 September 2014 1,613 2 View

What is the Pka of (Hydroxypropyl)methyl cellulose) and how do I prepare the 1:1 stoichiometry of 1 mM soln of HPMC with NaOH soln?

I would like to prepare 1mM stock solution of HPMC with NaOH. The ratio of -CH-OH in HPMC and Na+ should be in 1:1 ratio. Please help me to prepare this solution.

08 September 2014 1,649 0 View

How do I convert ug/ml biopolymer to a uM solution?

I purchased poly dA-dT.poly dA-dT 10 unit packs from sigma and the concentration was found spectrophotometrically. I got a weight of this product in ug/ ml but I would like to prepare a uM...

08 September 2014 2,486 6 View

Can anybody help me to prepare 3x 10 -4 concentrated solution of carboxymethyl cellulose polymer avg Mw 250,000?

Can anybody help me to prepare 3x 10 -4 concentrated solution of carboxymethyl cellulose polymer( avg Mw 250,000)

06 July 2014 1,531 5 View

How can I prepare 0.3 mM of Sodium carboxymethyl cellulose, average Mw ~250,000?

Will it follow the same molarity calculation as we do for small molecules, or is their any different calculations?

06 July 2014 7,322 1 View

What are the long-term impacts of incarceration on youths' developing brain?

I want to explore the long-term effects of incarceration on a youth's developing brain. I also want to explore research that looks critically at incarceration and punitive measures as the primary...

12 August 2024 862 0 View

• What the possible Persistent Organic Pollutants and Heavy metals present in fluorspar, sediments, and water bodies around its mining area?

Approximate concentrations are require in compared with the WHO permissible limts

11 August 2024 2,723 1 View

Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

11 August 2024 9,801 2 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Basis set and input instruction to calculate HOMO and LUMO using Orca for Imidazolium based organic salts?

Hey there, As a synthetic chemist delving into theoretical calculations for my imidazolium-based organic molecules, I would greatly appreciate any guidance on the appropriate input instructions...

09 August 2024 5,444 7 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

How to calculate CCS for Sodiated adduct ions and Multiply Charged Ions?

I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...

08 August 2024 8,329 0 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View