What is the proper range of FRET efficacy?

I would like to use FRET effect to probe the distance between two functional moieties (I am sure the distance is smaller than 10 nm). I have tried several times, but I found out that the FRET...

21 February 2021 3,562 1 View

Where can I buy Agilent software?

Hello. I have old agilent instrument, 6340 with 1100series HPLC, LC-Ion trap MS. We have software for LC, chemstation, but, not for MS. I asked Agilent korea if I can buy some software, and they...

05 January 2021 1,970 3 View

What's the function of each ingredient in a binding buffer?

I read a binding buffer in references. The recipe was listed as follows: Glucose (4.5g/L), Magnesium Chloride (5 mM), yeast tRNA (0.1 mg/ml) and BSA (1 mg/ml) was dissolved in DPBS. This buffer...

23 December 2020 8,440 4 View

Why transwell insert can cut off some drug molecules?

I am preparing a co-culture experiment in which transwell inserts will be used. According to the references, 0.4 micrometer PET membrane was selected. And different drugs were added to the upper...

15 November 2020 3,718 2 View

Can I use HUVEC cell line, not primary cell, to form barrier system in transwell?

I would like to measure the permeability of my drug using in vitro model, transwell, specifically. However, I only have the cell line, not the primary cell. Can I use the cell line to plate in the...

08 November 2020 3,330 1 View

How can I choose the concentration of Triton X-100 when I would like to permeabilize cells for flow cytometry?

Triton X-100 has been widely used to permeablize cells for flow cytometry. How can I choose the concentration of Triton X-100 if I would like to detect expression of one protein in the whole cell?...

31 October 2020 462 1 View

How to define Darcy's flow for pore pressure cohesive element in Abaqus?

Hi everyone, I am trying to define a Darcy's flow for pore pressure cohesive element in Abaqus. When I give permeability to the elements, the output of tangential flow rate (GFVR) is always shown...

11 October 2020 1,195 3 View

Extracting surface area, volume and cortical thickness from multiple groups using FreeSurfer?

Hello everyone, I'm new to FreeSurfer and this question might sound a bit stupid: I'm trying to get the volumetric measures, surface area and cortical thickness of brain regions of three groups...

29 July 2020 8,574 3 View

How many cm deepth of soil does the definition of soil composition generally refer to?

In general, for example, we think soil consist of 40% of minerals, 10% of organic matters, and 50% of water and air. My question is as follow: Does the soil used for the above definition refer...

03 July 2020 4,972 1 View

What are the dominate compounds of clay, silt, and sand in soil?

For example, what are the dominate 5 compounds in clay mineral? 80% of SiO2, etc.? Is the proportion/order of these dominate compounds changed with other factors, e.g., pH? Thank you in advance.

03 July 2020 453 1 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View