How to perform zymography of Acetyl Xylan esterase?

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Baydaa A. Hassan

I agree change the buffer solution that used

Maurice Ekpenyong

Zymogram analysis is performed by running PAGE gels of enzyme samples under native but reducing conditions according to the Bio-rad's manufacturer’s instructions. After being run, the gels are equilibrated in 10 mM phosphate buffer, pH 6.7, for 15 min, after which substrate-coupler solution by was added. Please see the attached for details. Article Characterization of an Acetyl Xylan Esterase from the Anaero...

and https://www.ajol.info/index.php/ajb/article/viewFile/126419/115934