Unlike xylan agar plates used for screening xylanase enzyme producers, is there any plate assay for screening beta xylosidase and arabinofuranosidase enzyme producers? In most of the work growing bacterial culture in broth and checking for enzyme activity is mentioned but at preliminary state how to select bacterial culture (in case working with lot of cultures).
Pankaj: How about using 4MU-derivatives of beta-xyloside and arabinoside, in which liberated 4MU can be detected by fluorescence, in 96-well plates?
You could also use 5-bromo-indolyl α-l-arabinofuranosyl and 5-Bromo-4-chloro-3-indolyl β-D-xylopyranoside. Compared to the above mentioned 4MU-derivatives, this would allow you to screen for the activity directly on plate, the positive colonies appearing in Blue, easier to scale-up than fluorescence on 96 well plates.
However, keep in mind that it presents a similar limitation than the 4MU-derivatives: it can only detect extracellular exo-acting enzymes.
Hi Pankaj,
As indicated by David, the plate method with X-substrates works extremely well, we have used it several times to detect arabinofurnanosidase and xylosidase activity, described in the papers attached.
Br
Thank you very much for all of your precious suggestions, that was really helpful.
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