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Questions related from Pankaj Parab
Hello, I am working on xylanolytic enzyme. During my recent study, in which I was trying to find for how long enzyme could be stable in buffer solutions (pH-5 to pH-9), I found that in some buffer...
09 September 2019 6,370 3 View
I'm working on purification of acetyl xylan esterase, I collected active fractions and wanted to confirm it's molecular weight by zymography, I am not getting single bands on SDS-PAGE by silver...
01 January 2019 7,666 2 View
I am trying to purify extracellular enzyme from culture supernatant, for that reason I concentrated SN and performed ion exchange chromatography using manual method (NaCl gradient in buffer,...
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I have gone through literature regarding colorimetric assay for AXE, but very few I could find. Some uses 4MUB derivatives and in some pNA, apart from these in one protocol bromothymol blue is...
05 May 2018 1,367 3 View
Unlike xylan agar plates used for screening xylanase enzyme producers, is there any plate assay for screening beta xylosidase and arabinofuranosidase enzyme producers? In most of the work growing...
03 March 2018 1,570 4 View
I'm trying to to purify protein sample using agilent HPLC system with C18 column. The moment I load sample and start program within few minutes the desired protein elute out along with solvent...
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