How to view the electrostatic potential value of a Gaussian09W result?

Hi all I am able to view the electrostatic potential surface using Gaussview to see the calculating result from Gaussian09W of a small molecule. However, the strength is represented by color. I am...

19 January 2021 5,448 3 View

Is K-silicate gel a porous material?

I coated a stone surface with K-siilcate. Apparently, it does noe seal the surface since the water penetrae the stone easily and the absorption coefficient does not change too much. However, the...

05 October 2020 3,435 5 View

Are there any reasech on loteprednol etabonate microemulsion or nanoemulsion?

The API (i.e. loteprednol etabonate )has poor solubility in water but has excellent solubility in Transcutol P which serves as cosurfactant in my microemulsion formulation. But when I added water...

24 September 2020 417 3 View

What type of graph is suitable for describing one variable and two dependent variables?

08 June 2020 9,347 3 View

How to analysis FTIR data?

02 January 2020 6,175 3 View

How to deprive ATP in cancer cell?

I want to increase chromatin compaction of cancer cell and to see the function of interested gene in this process. Is there any method to make it?

19 November 2019 5,972 1 View

What is the most suitable protocol to extract DNA from 70% ethanol fixed cell suspensions acquired from FACS?

I'm trying to extract DNA from 70% ethanol fixed cell suspensions. Firstly, The cells are fixed and stained for cell cycle analysis and collected after flow cytometry. the question is that the...

27 January 2019 9,767 3 View

Why the counterparts of virus A infectious clone swapped by counterparts of virus B cDNA fragments, virus A plasmid changed to low-copy?

does someone knows why the counterparts of virus A infectious clone swapped by same region of virus B cDNA fragments, virus A plasmid changed to low-copy?

14 January 2019 5,720 2 View

What is the most stable and accurate reference sample for conductive atomic force microscopy (CAFM)?

06 September 2017 1,995 2 View

Storage of exosomes

28 June 2017 5,137 2 View

Physics: Why do we describe light by referring to its wavelength, but microwaves and radar pulses by referring to their frequency?

In physics, the description of the properties of light generally refers to its wavelength (nm), while in the range of microwaves and radio waves we refer to the frequency of a signal (e.g. kHz or...

03 March 2021 1,682 3 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is there any effect of different colours of light (white, red, yellow, blue etc.) on the rate of photosynthesis? If yes, why?

Light is inevitable for the process of photosynthesis reaction. Naturally sunlight plays the vital role for this process. Light may be of different colours like white, red, yellow, blue etc....

02 March 2021 7,890 2 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

In an eye with Tyndall, does each dot correspond to an individual cell?

When you observe the presence of Tyndall in an inflamed eye: Does each of the small floating dots correspond to an individual cell or to an aggregate of cells? Does a conventional slit lamp have...

27 February 2021 6,326 2 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View

How can I obtain time series data by country on night-time light (brightness) ?

Hi everyone, Please, how can I obtain time series data by country on night-time light (brightness) ? I've tried, but so far all I can find are maps. I am looking for numerical data, especially in...

24 February 2021 8,625 4 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View